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Foetal calf serum or horse serum is added between the range 0.5–2% at the differentiation stage (Burattini, 2004; Chiron et al., 2012). Chicken embryo extract is also used as an addition to some cultures. Optimisation of the culture media is highly dependent on the cell species origin (Burton, Vierck, Krabbenhoft, Bryne, & Dodson, 2000). In ...
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