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  • Feeding the Newborn Dairy Calf - Penn State Extension

    2017-9-4u2002·u2002Figure 7. Calf survival rates by level of serum IgG. Source: USDA National Animal Health Monitoring System, 1993. Nutrient Requirements. The newborn calf must be fed highly digestible feedstuffs containing adequate levels of high-quality protein, energy, vitamins, and minerals. Protein. Protein provides amino acids used to build body tissues.

    Get Price
  • Cell Culture and Uses for Fetal Bovine Serum – Innovative ...

    Fetal bovine serum (FBS) is a product used in research laboratories, a blood product which exceeds cell culture testing standards and is used widely to promote growth medium. Because of its high quantity of hormones, carrier proteins and macromolecular proteins, fetal bovine serum is frequently present in culture medium used in in vitro fertilization.

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  • Probiotic Lactobacilli Administration Induces Changes in ...

    2021-8-14u2002·u2002One of the most critical challenges in the dairy industry is the high mortality rate of calves, which affects the replacement of heifers. Despite preventive measures, enteric infections and neonatal calf diarrhea (NCD) are among the leading causes of newborn deaths [1, 2].It is estimated that 5–6% of female dairy calves die during the preweaning period in farms in Canada and the USA [].

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  • Long-term growth comparison studies of FBS and FBS ...

    2017-6-7u2002·u2002Fetal bovine serum (FBS) is depended upon by investigators as an indispensable supplement in cell and tissue culture systems. Due to increased demand and limited availability, the price of FBS has increased by greater than 300% in the past few years. In addition, there are ethical and scientific controversies about the collection and use of FBS in culture systems.

    Get Price
  • Multiomic analysis of stretched osteocytes reveals ...

    2021-6-7u2002·u2002MLO-Y4: This cell line was acquired from Kerafast (EKC002) and expanded in MEM-α (Fisher) supplemented with 2.5% foetal calf serum (FBS) and 2.5% newborn calf serum …

    Get Price
  • Disease Prevention For Calves

    Disease Prevention For Calves. Zoetis provides a diverse portfolio of animal health products for Australian dairy herds. We also work with dairy farmers as well as veterinarians to help them make informed decisions that contribute to improving the health of their animals, and maximizing the productivity and profitability of their operations under sustainable conditions.

    Get Price
  • ERBB2 Increases Metastatic Potentials Specifically in ...

    2014-6-17u2002·u2002Despite all the blood-based biomarkers used to monitor prostate cancer patients, prostate cancer remains as the second common cause of cancer mortality in men in the United States. This is largely due to a lack of understanding of the molecular pathways that are responsible for the aggressive forms of prostate cancers, the castrate-resistant prostate cancer and the metastatic prostate cancer.

    Get Price
  • In Vitro and In Vivo Interspecies Chimera Assay Using ...

    2020-6-3u2002·u2002Introduction. The successful introduction of defined transcription factors into somatic cells yielded induced pluripotent stem cells (iPSCs) that are similar to embryonic stem cells (ESCs) with regard to major pluripotent characteristics, including unlimited self-renewal in vitro and the potential to differentiate into all somatic cells, and germ cells, in vitro and in vivo (Choi et al., 2016 ...

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  • Ptch2/Gas1 and Ptch1/Boc differentially regulate

    2020-4-24u2002·u2002Gas1 and Boc/Cdon act as co-receptors in the vertebrate Hedgehog signalling pathway, but the nature of their interaction with the primary Ptch1/2 receptors remains unclear. Here we demonstrate ...

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  • Long-term growth comparison studies of FBS and FBS ...

    2017-6-7u2002·u2002Fetal bovine serum (FBS) is depended upon by investigators as an indispensable supplement in cell and tissue culture systems. Due to increased demand and limited availability, the price of FBS has increased by greater than 300% in the past few years. In addition, there are ethical and scientific controversies about the collection and use of FBS in culture systems.

    Get Price
  • IgG Subclasses and Allotypes: From Structure to Effector ...

    2014-10-20u2002·u2002IgG1 . Antibody responses to soluble protein antigens and membrane proteins primarily induce IgG1, but are accompanied with lower levels of the other subclasses, mostly IgG3 IgG4 ().Because IgG1 is normally the most abundant subclass, a lack of IgG1 seen in a variety of primary and secondary antibody deficiencies, can result in decreased total IgG levels …

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  • MSCs Conditioned Media and Umbilical Cord Blood Plasma ...

    2014-11-25u2002·u2002Human mesenchymal stem cells (hMSCs) from umbilical cord (UC) blood (UCB) and matrix are tested clinically for a variety of pathologies but in vitro expansion using culture media containing fetal bovine serum (FBS) is essential to achieve appropriate cell numbers for clinical use. Human UCB plasma (hUCBP) can be used as a supplement for hMSCs culture, since UCB is rich …

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  • Multiomic analysis of stretched osteocytes reveals ...

    2021-6-7u2002·u2002MLO-Y4: This cell line was acquired from Kerafast (EKC002) and expanded in MEM-α (Fisher) supplemented with 2.5% foetal calf serum (FBS) and 2.5% newborn calf serum (NCS) both inactivated (Fisher ...

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  • A lentiviral system for efficient knockdown...

    2017-12-5u2002·u2002We maintain HEK-293-T cells in DMEM high glucose medium supplemented with 10% iron-supplemented calf serum at 37°C in an 5% CO 2-containing, water-saturated atmosphere. To improve cell survival during virus production, the culture medium can be supplemented with 1x non-essential amino acids and 1 mM sodium pyruvate (optional).

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  • Disease Prevention For Calves

    Disease Prevention For Calves. Zoetis provides a diverse portfolio of animal health products for Australian dairy herds. We also work with dairy farmers as well as veterinarians to help them make informed decisions that contribute to improving the health of their animals, and maximizing the productivity and profitability of their operations under sustainable conditions.

    Get Price
  • Analyses of basal media and serum for in vitro expansion ...

    2015-8-1u2002·u2002Transplantation of stem cells requires a huge amount of cells, deeming the expansion of the cells in vitro necessary. The aim of this study is to define the optimal combination of basal medium and serum for the expansion of suspension peripheral blood mononucleated stem cells (PBMNSCs) without resulting in loss in the differentiation potential. Mononucleated cells were …

    Get Price
  • In Vitro and In Vivo Interspecies Chimera Assay Using ...

    2020-6-3u2002·u2002Introduction. The successful introduction of defined transcription factors into somatic cells yielded induced pluripotent stem cells (iPSCs) that are similar to embryonic stem cells (ESCs) with regard to major pluripotent characteristics, including unlimited self-renewal in vitro and the potential to differentiate into all somatic cells, and germ cells, in vitro and in vivo (Choi et al., …

    Get Price
  • SIRT6 deficiency results in developmental retardation in ...

    2018-8-22u2002·u2002A cynomolgus monkey model deficient in SIRT6 protein exhibits severe retardation in prenatal development, in which neuronal differentiation is delayed by activation of the H19 long non-coding RNA.

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  • Soft Tissue Repair with Easy-Accessible Autologous Newborn ...

    2017-12-17u2002·u2002Disrupted organogenesis leads to permanent malformations that may require surgical correction. Autologous tissue grafts may be needed in severe lack of orthotopic tissue but include donor site morbidity. The placenta is commonly discarded after birth and has a therapeutic potential. The aim of this study was to determine if the amnion from placenta or plasma rich of …

    Get Price
  • Feeding the Newborn Dairy Calf - Penn State Extension

    2017-9-4u2002·u2002Figure 7. Calf survival rates by level of serum IgG. Source: USDA National Animal Health Monitoring System, 1993. Nutrient Requirements. The newborn calf must be fed highly digestible feedstuffs containing adequate levels of high-quality protein, energy, vitamins, and minerals. Protein. Protein provides amino acids used to build body tissues.

    Get Price
  • Serum alternatives and engineered sera

    Engineered serum products and non-fetal cell culture alternatives including bovine calf serum (BCS) act as a cost-effective replacement for FBS while ensuring high performance. Engineered serum and bovine serum alternatives FAQs. Below are answers to some common questions regarding engineered serum and FBS alternatives.

    Get Price
  • Probiotic Lactobacilli Administration Induces Changes in ...

    2021-8-14u2002·u2002One of the most critical challenges in the dairy industry is the high mortality rate of calves, which affects the replacement of heifers. Despite preventive measures, enteric infections and neonatal calf diarrhea (NCD) are among the leading causes of newborn deaths [1, 2].It is estimated that 5–6% of female dairy calves die during the preweaning period in farms in Canada …

    Get Price
  • Disease Prevention For Calves

    Disease Prevention For Calves. Zoetis provides a diverse portfolio of animal health products for Australian dairy herds. We also work with dairy farmers as well as veterinarians to help them make informed decisions that contribute to improving the health of their animals, and maximizing the productivity and profitability of their operations under sustainable conditions.

    Get Price
  • ERBB2 Increases Metastatic Potentials Specifically in ...

    2014-6-17u2002·u2002Despite all the blood-based biomarkers used to monitor prostate cancer patients, prostate cancer remains as the second common cause of cancer mortality in men in the United States. This is largely due to a lack of understanding of the molecular pathways that are responsible for the aggressive forms of prostate cancers, the castrate-resistant prostate cancer and the …

    Get Price
  • Soft Tissue Repair with Easy-Accessible Autologous Newborn ...

    2017-12-17u2002·u2002Disrupted organogenesis leads to permanent malformations that may require surgical correction. Autologous tissue grafts may be needed in severe lack of orthotopic tissue but include donor site morbidity. The placenta is commonly discarded after birth and has a therapeutic potential. The aim of this study was to determine if the amnion from placenta or plasma rich of …

    Get Price
  • In Vitro Culturing of Adult Stem Cells: The Importance of ...

    2021-8-24u2002·u2002Mannello F, Tonti GA (2007) Concise review: no breakthroughs for human mesenchymal and embryonic stem cell culture: conditioned medium, feeder layer, or feeder-free; medium with fetal calf serum, human serum, or enriched plasma; serum-free, serum replacement nonconditioned medium, or ad hoc formula? All that glitters is not gold.

    Get Price
  • GH3, a novel proapoptotic domain in Drosophila Grim ...

    2002-7-1u2002·u2002We tested whether GH3 was important for Grim proapoptotic function by assaying the cell killing ability of several Grim mutants altered in the GH3 domain in Drosophila SL2 cells (Figure 2 A). As previously described (Chen et al., 1996b), wild-type (WT) Grim induced cell death when overexpressed in this assay (Figure 2 B).In contrast, a Grim mutant form with a 13 amino acid …

    Get Price
  • EMBRYONIC S C :CHARACTERIZATION S - Wiley

    Activin A in the presence of low fetal calf serum (FCS). The development of hESCs into DE by this approach closely follows the series of events associated with endoderm formation during vertebrate development. Understanding the developmental pathways controlling DE formation at the molecular level has so far been guided by

    Get Price
  • Collagen overlays can inhibit leptin and adiponectin ...

    2018-4-13u2002·u2002Background White adipose tissue (WAT) is essential for energy storage as well as being an active endocrine organ. The secretion of adipokines by adipocytes can affect whole body metabolism, appetite, and contribute to overall health. WAT is comprised of lipid-laden mature adipocytes, as well as immune cells, endothelial cells, pre-adipocytes, and adipose-derived stem …

    Get Price
  • Feeding the Newborn Dairy Calf - Penn State Extension

    2017-9-4u2002·u2002Figure 7. Calf survival rates by level of serum IgG. Source: USDA National Animal Health Monitoring System, 1993. Nutrient Requirements. The newborn calf must be fed highly digestible feedstuffs containing adequate levels of high-quality protein, energy, vitamins, and minerals. Protein. Protein provides amino acids used to build body tissues.

    Get Price
  • An Arginine-Faced Amphipathic Alpha Helix Is Required

    HeLa (CCL2.2) and W162 cells were maintained in Dulbecco modified Eagle's minimal medium supplemented with 10% newborn calf serum as previously described . dl309 served as the wild-type Ad5 used in these studies. dl309 lacks a portion of the E3 gene which has been shown to be dispensable for growth in culture .

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  • Making the Switch: Alternatives to Fetal Bovine Serum for ...

    Human AB serum and thrombin-activated platelet-rich plasma are suitable alternatives to fetal calf serum for the expansion of mesenchymal stem cells from adipose tissue. Stem Cells 25, 1270–1278. doi: 10.1634/stemcells.2006-0627

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  • Changes in Culture Expanded Human Amniotic Epithelial ...

    2011-11-2u2002·u2002Human amniotic epithelial cells (hAEC) isolated from term placenta have stem cell-like properties, differentiate into tissue specific cells and reduce lung and liver inflammation and fibrosis following transplantation into disease models established in mice. These features together with their low immunogenicity and immunosuppressive properties make hAEC an attractive source of cells for ...

    Get Price
  • Multiomic analysis of stretched osteocytes reveals ...

    2021-6-7u2002·u2002MLO-Y4: This cell line was acquired from Kerafast (EKC002) and expanded in MEM-α (Fisher) supplemented with 2.5% foetal calf serum (FBS) and 2.5% newborn calf serum …

    Get Price
  • Disease Prevention For Calves

    Disease Prevention For Calves. Zoetis provides a diverse portfolio of animal health products for Australian dairy herds. We also work with dairy farmers as well as veterinarians to help them make informed decisions that contribute to improving the health of their animals, and maximizing the productivity and profitability of their operations under sustainable conditions.

    Get Price
  • Analyses of basal media and serum for in vitro expansion ...

    2015-8-1u2002·u2002Transplantation of stem cells requires a huge amount of cells, deeming the expansion of the cells in vitro necessary. The aim of this study is to define the optimal combination of basal medium and serum for the expansion of suspension peripheral blood mononucleated stem cells (PBMNSCs) without resulting in loss in the differentiation potential. Mononucleated cells were isolated from both mice ...

    Get Price
  • ERBB2 Increases Metastatic Potentials Specifically in ...

    2014-6-17u2002·u2002Despite all the blood-based biomarkers used to monitor prostate cancer patients, prostate cancer remains as the second common cause of cancer mortality in men in the United States. This is largely due to a lack of understanding of the molecular pathways that are responsible for the aggressive forms of prostate cancers, the castrate-resistant prostate cancer and the metastatic prostate cancer.

    Get Price
  • iHerb.com - Vitamins, Supplements & Natural Health

    Giovanni, Frizz Be Gone, Super Smoothing, Anti-Frizz Hair Serum, 2.75 fl oz (81 ml) 14230. $6.44 $7.16. Add to cart. Azelique, Serumdipity, Age Refining Hyaluronic Acid, Facial Serum, 1 fl oz (30 ml) 6072. $10.00 $20.00. Add to cart. Ariul, Stress Relieving Hand Sanitizing Tissue, 99.9% Efficacy, 80 Sheets, 10.82 fl oz (320 ml) 16198. $2.11 $3 ...

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  • Clinical Scenarios Flashcards

    If the fasting glucose is greater than 126 mg/dL, OR the one-hour glucose is greater than 130mg/dL (90% sensitivity) or 140mg/dL (80% sensitivity), then the patient is considered to have a positive result. In the case of a positive one-hour glucose, the patient should undergo a …

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  • Feeding the Newborn Dairy Calf - Penn State Extension

    2017-9-4u2002·u2002Figure 7. Calf survival rates by level of serum IgG. Source: USDA National Animal Health Monitoring System, 1993. Nutrient Requirements. The newborn calf must be fed highly digestible feedstuffs containing adequate levels of high-quality protein, energy, vitamins, and minerals. Protein. Protein provides amino acids used to build body tissues.

    Get Price
  • NeuroCult™ SM1 Neuronal Culture Supplement

    NeuroCult™ SM1 may also be used as a serum-replacement supplement for various customizable applications, such as neurotoxicity assays and calcium imaging. For your convenience, NeuroCult™ SM1 is included as a component of multiple BrainPhys™ Neuronal Medium culture kits for primary and hPSC-derived neurons (Catalog #05792, 05793, 05794 ...

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  • Disease Prevention For Calves

    Disease Prevention For Calves. Zoetis provides a diverse portfolio of animal health products for Australian dairy herds. We also work with dairy farmers as well as veterinarians to help them make informed decisions that contribute to improving the health of their animals, and maximizing the productivity and profitability of their operations under sustainable conditions.

    Get Price
  • Culture and Characterization of Human Embryonic Stem

    2004-9-29u2002·u2002Originally, human ES cells were derived and maintained in medium containing fetal calf serum. They are now widely cultured in a proprietary serum-free formulation (serum replacement from Invitrogen Corp., Carlsbad, CA), and recently we have derived a new human ES line in this medium without fetal calf serum.

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  • Bio-Mos® Feed Supplement for Dairy Cattle, Beef Cattle ...

    Bio-Mos gut health and performance supplement from Alltech is a unique prebiotic designed to maximize gastrointestinal integrity in all stages of cattle growth. 25 kg. (55.1 lb.) Bag Benefits Supports the transfer of vital colostrum nutrients from cow to calf Contributes to immune system development and helps facilitat

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  • Changes in Culture Expanded Human Amniotic Epithelial ...

    2011-11-2u2002·u2002Human amniotic epithelial cells (hAEC) isolated from term placenta have stem cell-like properties, differentiate into tissue specific cells and reduce lung and liver inflammation and fibrosis following transplantation into disease models established in mice. These features together with their low immunogenicity and immunosuppressive properties make hAEC an attractive …

    Get Price
  • Life Technologies India: - FBS (foetal bovine serum, Fetal ...

    2016-11-9u2002·u2002Life Technologies (India) Pvt. Ltd. is a supplier of products & services to life science research, industrial biotech & pharma sector in India and neighbouring countries - in the fields of Immunology, Stem Cell Research, Human Cytogenetics, Phytodiagnostics, Tissue Culture, Bio-safety, Bio-stability, Bio-defense, Discovery Biology including GMO testing.

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  • Effects of Neuropeptide Y on Stem Cells and Their ...

    Neuropeptide Y (NPY), a 36-amino acid peptide, is widely distributed in the central and peripheral nervous systems and other peripheral tissues. It takes part in regulating various biological processes including food intake, circadian rhythm, energy metabolism, and neuroendocrine secretion. Increasing evidence indicates that NPY exerts multiple regulatory effects on stem cells.

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  • ERBB2 Increases Metastatic Potentials Specifically in ...

    2014-6-17u2002·u2002Despite all the blood-based biomarkers used to monitor prostate cancer patients, prostate cancer remains as the second common cause of cancer mortality in men in the United States. This is largely due to a lack of understanding of the molecular pathways that are responsible for the aggressive forms of prostate cancers, the castrate-resistant prostate cancer …

    Get Price
  • THE SKINBUREAU SKINCARE ENTERPRISES

    Hand Sanitizer. The Skin Bureau Hand Sanitizer is composed of 70% Ethyl Alcohol and Vitamin E used to disinfect and nourish the skin. BENEFITS. • Tough on dirt, gentle on skin. • Compact and handy. • Scented Hand Sanitizer. • Kills 99.9% of germs in contact. Buy Now.

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  • Feeding the Newborn Dairy Calf - Penn State Extension

    2017-9-4u2002·u2002Figure 7. Calf survival rates by level of serum IgG. Source: USDA National Animal Health Monitoring System, 1993. Nutrient Requirements. The newborn calf must be fed highly digestible feedstuffs containing adequate levels of high-quality protein, energy, vitamins, and minerals. Protein. Protein provides amino acids used to build body tissues.

    Get Price
  • Serum alternatives and engineered sera

    Engineered serum products and non-fetal cell culture alternatives including bovine calf serum (BCS) act as a cost-effective replacement for FBS while ensuring high performance. Engineered serum and bovine serum alternatives FAQs. Below are answers to some common questions regarding engineered serum and FBS alternatives.

    Get Price
  • Probiotic Lactobacilli Administration Induces Changes in ...

    2021-8-14u2002·u2002One of the most critical challenges in the dairy industry is the high mortality rate of calves, which affects the replacement of heifers. Despite preventive measures, enteric infections and neonatal calf diarrhea (NCD) are among the leading causes of newborn deaths [1, 2].It is estimated that 5–6% of female dairy calves die during the preweaning period in farms in …

    Get Price
  • Disease Prevention For Calves

    Disease Prevention For Calves. Zoetis provides a diverse portfolio of animal health products for Australian dairy herds. We also work with dairy farmers as well as veterinarians to help them make informed decisions that contribute to improving the health of their animals, and maximizing the productivity and profitability of their operations under sustainable conditions.

    Get Price
  • ERBB2 Increases Metastatic Potentials Specifically in ...

    2014-6-17u2002·u2002Despite all the blood-based biomarkers used to monitor prostate cancer patients, prostate cancer remains as the second common cause of cancer mortality in men in the United States. This is largely due to a lack of understanding of the molecular pathways that are responsible for the aggressive forms of prostate cancers, the castrate-resistant prostate cancer …

    Get Price
  • Soft Tissue Repair with Easy-Accessible Autologous

    2017-12-17u2002·u2002Disrupted organogenesis leads to permanent malformations that may require surgical correction. Autologous tissue grafts may be needed in severe lack of orthotopic tissue but include donor site morbidity. The placenta is commonly discarded after birth and has a therapeutic potential. The aim of this study was to determine if the amnion from placenta or plasma rich of …

    Get Price
  • In Vitro Culturing of Adult Stem Cells: The Importance of ...

    2021-8-24u2002·u2002Mannello F, Tonti GA (2007) Concise review: no breakthroughs for human mesenchymal and embryonic stem cell culture: conditioned medium, feeder layer, or feeder-free; medium with fetal calf serum, human serum, or enriched plasma; serum-free, serum replacement nonconditioned medium, or ad hoc formula? All that glitters is not gold.

    Get Price
  • GH3, a novel proapoptotic domain in Drosophila Grim ...

    2002-7-1u2002·u2002We tested whether GH3 was important for Grim proapoptotic function by assaying the cell killing ability of several Grim mutants altered in the GH3 domain in Drosophila SL2 cells (Figure 2 A). As previously described (Chen et al., 1996b), wild-type (WT) Grim induced cell death when overexpressed in this assay (Figure 2 B).In contrast, a Grim mutant form with a 13 amino …

    Get Price
  • EMBRYONIC S C :CHARACTERIZATION S - Wiley

    Activin A in the presence of low fetal calf serum (FCS). The development of hESCs into DE by this approach closely follows the series of events associated with endoderm formation during vertebrate development. Understanding the developmental pathways controlling DE formation at the molecular level has so far been guided by

    Get Price
  • Collagen overlays can inhibit leptin and adiponectin ...

    2018-4-13u2002·u2002Background White adipose tissue (WAT) is essential for energy storage as well as being an active endocrine organ. The secretion of adipokines by adipocytes can affect whole body metabolism, appetite, and contribute to overall health. WAT is comprised of lipid-laden mature adipocytes, as well as immune cells, endothelial cells, pre-adipocytes, and adipose-derived …

    Get Price