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Get Price2016-3-18u2002·u2002In conclusion, we demonstrated our primary goal: the necessity of sufficient oxygen supply by Hb for efficient PDT against hypoxic tumors, and the self …
Get Price2008-2-1u2002·u2002Therefore, immortalized human liver cell lines with sufficient in vitro hepatic functionality are considered most suitable for application in cell-based, liver support treatments . Recently, we have developed a new cell line by immortalization of human fetal liver cells after overexpressing human telomerase reverse transcriptase.
Get Price2019-10-29u2002·u2002Maternal Fasting Rescues Metabolic Dysfunction in Mpc1-Deficient Fetuses. To better characterize the nutrient allocation between the maternal and fetal compartments, we compared steady-state maternal serum metabolites and maternal liver and fetal liver metabolites by 1 H-NMR metabolomics. Consistent with the decrease in blood glucose and increase in serum β …
Get Price2011-1-6u2002·u2002Fibrinogen is a hexamer composed of 2 copies of 3 polypeptide chains Aα, Bβ and γ. These are encoded by 3 genes, FGB, FGA, and FGG (ordered from centromere to telomere) clustered in a 50-kb region on the long arm of human chromosome 4 (4q32). Fibrinogen biosynthesis occurs in the liver where the 3 genes undergo coordinate transcription.
Get Price2021-7-23u2002·u2002Total RNA purification was conducted by direct-zol RNA miniprep (zymo research, Irvine, CA) or RNeasy mini kit (Hilden, Germany) from a cell line or …
Get Price2021-1-1u2002·u2002The liver is one of the few solid organs with a high regeneration capacity. After partial hepatectomy (PHx) or chemical challenge in the mammalian liver, almost all remnant hepatocytes rapidly initiate mitosis to generate new cells, and the liver can be reconstituted within 1 week. 1, 2 Many genes and signaling pathways are involved in this process. . For example, immediately after PHx ...
Get Price2020-1-17u2002·u2002In the bovine placenta, intimate fetomaternal contacts are restricted to discrete placentomes. Here, widely branched fetal chorionic villi interdigitate with corresponding maternal caruncular crypts. The fetal trophoblast epithelium covering the chorionic villi consists of approximately 80% uninucleate trophoblast cells (UTCs) and 20% binuclear trophoblast giant cells (TGCs).
Get Price2019-9-10u2002·u2002Cholestatic liver diseases are characterized by impaired bile formation, resulting in hepatic accumulation of BA, cholesterol, and bilirubin 24; once BAs accumulate in high concentrations, they can leak from canaliculi and bile ducts, thus causing hepatic cell death, inflammation, fibrosis, and cancer. 25 Cholestatic liver diseases are also ...
Get Price2012-6-7u2002·u2002It has been reported that the phosphatidylinositol 3-kinase (PI3K)-AKT signaling pathway regulates erythropoietin (EPO)-induced survival, proliferation, and maturation of early erythroid progenitors. Erythroid cell proliferation and survival have also been related to activation of the JAK-STAT pathway. The goal of this study was to observe the function of EPO activation of JAK-STAT and PI3K ...
Get Price2016-3-18u2002·u2002MCF-7 cells were cultured in Dulbecco's modified eagle medium (DMEM) supplemented with 10% fetal bovine serum (FBS) and 1% of penicillin and streptomycin. For in vitro detection of ROS generation, MCF-7 cells were incubated with DCFH-DA (10 μM) along with INPs or I-ARCs for 30 min, according to the manufacturer's suggested protocol.
Get Price2021-9-19u2002·u2002Hepatocyte seeding and culture medium. William's medium E containing 10 % fetal bovine serum (FBS), suitable antibiotics and 2 ng/mL insulin. Media should be warmed to 37 °C before use (see Note 1). Phosphate-buffered saline (PBS). 137 mM NaCl, 2.7 mM KCl, 10 mM Na 2 HPO 4 ·2H 2 O, 1.8 mM KH 2 PO 4 in deionized water. Adjust to pH 7.4 and ...
Get Price2016-3-18u2002·u2002In conclusion, we demonstrated our primary goal: the necessity of sufficient oxygen supply by Hb for efficient PDT against hypoxic tumors, and the self …
Get Price2011-1-6u2002·u2002Fibrinogen is a hexamer composed of 2 copies of 3 polypeptide chains Aα, Bβ and γ. These are encoded by 3 genes, FGB, FGA, and FGG (ordered from centromere to telomere) clustered in a 50-kb region on the long arm of human chromosome 4 (4q32). Fibrinogen biosynthesis occurs in the liver where the 3 genes undergo coordinate transcription.
Get Price2008-10-1u2002·u2002The cells were plated at a concentration of 10 5 cells/mL in Dulbecco's modified Eagle's medium with 10% fetal bovine serum, 2 mM L-glutamine, 100 units/mL penicillin, 10 µg/mL streptomycin (Invitrogen). Twenty percent fetal bovine serum was used for culture of FBHE and CPAE cells.
Get Price2021-1-1u2002·u2002The liver is one of the few solid organs with a high regeneration capacity. After partial hepatectomy (PHx) or chemical challenge in the mammalian liver, almost all remnant hepatocytes rapidly initiate mitosis to generate new cells, and the liver can be reconstituted within 1 week. 1, 2 Many genes and signaling pathways are involved in this process. . For example, immediately after PHx ...
Get Price2020-2-15u2002·u2002Islet transplantation is a promising technique for treating patients with diabetes. The liver is the current site of choice for clinical islet transplantation, but the major disadvantage of islet transplantation in this site is that many intraportal grafts are lost or damaged in the early stage of transplantation [1, 2].Additional disadvantages include the difficulty of monitoring the islets ...
Get Price2021-4-6u2002·u2002The cells were grown in RPMI 1640 medium (HyClone, Logan, UT) supplemented with 10% (v/v) fetal bovine serum, 100 U/ml of penicillin, and 100 mg/ml of streptomycin at 37 °C in 5% (v/v) CO 2 atmosphere. The uptake of ATV and GCDCA-S were measured using ATV and GCDCA-S (10 µM) in the presence or absence of RIF (0.1, 0.5, 1, 5, 10 and 50 µM).
Get Price2019-5-13u2002·u2002human fetal liver (FL), cord blood (CB), and adult bone marrow (BM) enhance CD34+ hematopoietic progenitor cell proliferation and erythropoiesis [19]. We have exam-ined the growth and erythroid differentiation capacity of CD34+ cells only, already committed toward hematopoiesis in fetal and adult hematopoietic cells.
Get Price2019-9-10u2002·u2002Cholestatic liver diseases are characterized by impaired bile formation, resulting in hepatic accumulation of BA, cholesterol, and bilirubin 24; once BAs accumulate in high concentrations, they can leak from canaliculi and bile ducts, thus causing hepatic cell death, inflammation, fibrosis, and cancer. 25 Cholestatic liver diseases are also ...
Get Price2016-3-18u2002·u2002MCF-7 cells were cultured in Dulbecco's modified eagle medium (DMEM) supplemented with 10% fetal bovine serum (FBS) and 1% of penicillin and streptomycin. For in vitro detection of ROS generation, MCF-7 cells were incubated with DCFH-DA (10 μM) along with INPs or I-ARCs for 30 min, according to the manufacturer's suggested protocol.
Get Price2021-9-19u2002·u2002Hepatocyte seeding and culture medium. William's medium E containing 10 % fetal bovine serum (FBS), suitable antibiotics and 2 ng/mL insulin. Media should be warmed to 37 °C before use (see Note 1). Phosphate-buffered saline (PBS). 137 mM NaCl, 2.7 mM KCl, 10 mM Na 2 HPO 4 ·2H 2 O, 1.8 mM KH 2 PO 4 in deionized water. Adjust to pH 7.4 and ...
Get Price2016-3-18u2002·u2002In conclusion, we demonstrated our primary goal: the necessity of sufficient oxygen supply by Hb for efficient PDT against hypoxic tumors, and the self-monitoring PDT through FL/PA dual-modal imaging.
Get Price2011-1-6u2002·u2002Fibrinogen is a hexamer composed of 2 copies of 3 polypeptide chains Aα, Bβ and γ. These are encoded by 3 genes, FGB, FGA, and FGG (ordered from centromere to telomere) clustered in a 50-kb region on the long arm of human chromosome 4 (4q32). Fibrinogen biosynthesis occurs in the liver where the 3 genes undergo coordinate transcription.
Get Price2008-10-1u2002·u2002The cells were plated at a concentration of 10 5 cells/mL in Dulbecco's modified Eagle's medium with 10% fetal bovine serum, 2 mM L-glutamine, 100 units/mL penicillin, 10 µg/mL streptomycin (Invitrogen). Twenty percent fetal bovine serum was …
Get Price2021-1-1u2002·u2002The liver is one of the few solid organs with a high regeneration capacity. After partial hepatectomy (PHx) or chemical challenge in the mammalian liver, almost all remnant hepatocytes rapidly initiate mitosis to generate new cells, and the liver can be reconstituted within 1 week. 1, 2 Many genes and signaling pathways are involved in this process. . For example, immediately after …
Get Price2020-2-15u2002·u2002Islet transplantation is a promising technique for treating patients with diabetes. The liver is the current site of choice for clinical islet transplantation, but the major disadvantage of islet transplantation in this site is that many intraportal grafts are lost or damaged in the early stage of transplantation [1, 2].Additional disadvantages include the difficulty of monitoring the islets ...
Get Price2021-4-6u2002·u2002The cells were grown in RPMI 1640 medium (HyClone, Logan, UT) supplemented with 10% (v/v) fetal bovine serum, 100 U/ml of penicillin, and 100 mg/ml of streptomycin at 37 °C in 5% (v/v) CO 2 atmosphere. The uptake of ATV and GCDCA-S were measured using ATV and GCDCA-S (10 µM) in the presence or absence of RIF (0.1, 0.5, 1, 5, 10 and 50 µM).
Get Price2019-5-13u2002·u2002human fetal liver (FL), cord blood (CB), and adult bone marrow (BM) enhance CD34+ hematopoietic progenitor cell proliferation and erythropoiesis [19]. We have exam-ined the growth and erythroid differentiation capacity of CD34+ cells only, already committed toward hematopoiesis in fetal and adult hematopoietic cells.
Get Price2019-9-10u2002·u2002Cholestatic liver diseases are characterized by impaired bile formation, resulting in hepatic accumulation of BA, cholesterol, and bilirubin 24; once BAs accumulate in high concentrations, they can leak from canaliculi and bile ducts, thus causing hepatic cell death, inflammation, fibrosis, and cancer. 25 Cholestatic liver diseases are also ...
Get Price2016-3-18u2002·u2002MCF-7 cells were cultured in Dulbecco's modified eagle medium (DMEM) supplemented with 10% fetal bovine serum (FBS) and 1% of penicillin and streptomycin. For in vitro detection of ROS generation, MCF-7 cells were incubated with DCFH-DA (10 μM) along with INPs or I-ARCs for 30 min, according to the manufacturer's suggested protocol.
Get Price2021-9-19u2002·u2002Hepatocyte seeding and culture medium. William's medium E containing 10 % fetal bovine serum (FBS), suitable antibiotics and 2 ng/mL insulin. Media should be warmed to 37 °C before use (see Note 1). Phosphate-buffered saline (PBS). 137 mM NaCl, 2.7 mM KCl, 10 mM Na 2 HPO 4 ·2H 2 O, 1.8 mM KH 2 PO 4 in deionized water. Adjust to pH 7.4 and ...
Get Price2016-3-18u2002·u2002In conclusion, we demonstrated our primary goal: the necessity of sufficient oxygen supply by Hb for efficient PDT against hypoxic tumors, and the self …
Get Price2011-1-6u2002·u2002Fibrinogen is a hexamer composed of 2 copies of 3 polypeptide chains Aα, Bβ and γ. These are encoded by 3 genes, FGB, FGA, and FGG (ordered from centromere to telomere) clustered in a 50-kb region on the long arm of human chromosome 4 (4q32). Fibrinogen biosynthesis occurs in the liver where the 3 genes undergo coordinate transcription.
Get Price2008-10-1u2002·u2002The cells were plated at a concentration of 10 5 cells/mL in Dulbecco's modified Eagle's medium with 10% fetal bovine serum, 2 mM L-glutamine, 100 units/mL penicillin, 10 µg/mL streptomycin (Invitrogen). Twenty percent fetal bovine serum was used for culture of FBHE and CPAE cells.
Get Price2021-1-1u2002·u2002The liver is one of the few solid organs with a high regeneration capacity. After partial hepatectomy (PHx) or chemical challenge in the mammalian liver, almost all remnant hepatocytes rapidly initiate mitosis to generate new cells, and the liver can be reconstituted within 1 week. 1, 2 Many genes and signaling pathways are involved in this process. . For example, immediately after PHx ...
Get Price2020-2-15u2002·u2002Islet transplantation is a promising technique for treating patients with diabetes. The liver is the current site of choice for clinical islet transplantation, but the major disadvantage of islet transplantation in this site is that many intraportal grafts are lost or damaged in the early stage of transplantation [1, 2].Additional disadvantages include the difficulty of monitoring the islets ...
Get Price2021-4-6u2002·u2002The cells were grown in RPMI 1640 medium (HyClone, Logan, UT) supplemented with 10% (v/v) fetal bovine serum, 100 U/ml of penicillin, and 100 mg/ml of streptomycin at 37 °C in 5% (v/v) CO 2 atmosphere. The uptake of ATV and GCDCA-S were measured using ATV and GCDCA-S (10 µM) in the presence or absence of RIF (0.1, 0.5, 1, 5, 10 and 50 µM).
Get Price2019-5-13u2002·u2002human fetal liver (FL), cord blood (CB), and adult bone marrow (BM) enhance CD34+ hematopoietic progenitor cell proliferation and erythropoiesis [19]. We have exam-ined the growth and erythroid differentiation capacity of CD34+ cells only, already committed toward hematopoiesis in fetal and adult hematopoietic cells.
Get Price2019-9-10u2002·u2002Cholestatic liver diseases are characterized by impaired bile formation, resulting in hepatic accumulation of BA, cholesterol, and bilirubin 24; once BAs accumulate in high concentrations, they can leak from canaliculi and bile ducts, thus causing hepatic cell death, inflammation, fibrosis, and cancer. 25 Cholestatic liver diseases are also ...
Get Price2016-3-18u2002·u2002MCF-7 cells were cultured in Dulbecco's modified eagle medium (DMEM) supplemented with 10% fetal bovine serum (FBS) and 1% of penicillin and streptomycin. For in vitro detection of ROS generation, MCF-7 cells were incubated with DCFH-DA (10 μM) along with INPs or I-ARCs for 30 min, according to the manufacturer's suggested protocol.
Get Price2021-9-19u2002·u2002Hepatocyte seeding and culture medium. William's medium E containing 10 % fetal bovine serum (FBS), suitable antibiotics and 2 ng/mL insulin. Media should be warmed to 37 °C before use (see Note 1). Phosphate-buffered saline (PBS). 137 mM NaCl, 2.7 mM KCl, 10 mM Na 2 HPO 4 ·2H 2 O, 1.8 mM KH 2 PO 4 in deionized water. Adjust to pH 7.4 and ...
Get Price2016-3-18u2002·u2002In conclusion, we demonstrated our primary goal: the necessity of sufficient oxygen supply by Hb for efficient PDT against hypoxic tumors, and the self-monitoring PDT through FL/PA dual-modal imaging.
Get Price2011-1-6u2002·u2002Fibrinogen is a hexamer composed of 2 copies of 3 polypeptide chains Aα, Bβ and γ. These are encoded by 3 genes, FGB, FGA, and FGG (ordered from centromere to telomere) clustered in a 50-kb region on the long arm of human chromosome 4 (4q32). Fibrinogen biosynthesis occurs in the liver where the 3 genes undergo coordinate transcription.
Get Price2008-10-1u2002·u2002The cells were plated at a concentration of 10 5 cells/mL in Dulbecco's modified Eagle's medium with 10% fetal bovine serum, 2 mM L-glutamine, 100 units/mL penicillin, 10 µg/mL streptomycin (Invitrogen). Twenty percent fetal bovine serum was …
Get Price2021-1-1u2002·u2002The liver is one of the few solid organs with a high regeneration capacity. After partial hepatectomy (PHx) or chemical challenge in the mammalian liver, almost all remnant hepatocytes rapidly initiate mitosis to generate new cells, and the liver can be reconstituted within 1 week. 1, 2 Many genes and signaling pathways are involved in this process. . For example, immediately after …
Get Price2020-2-15u2002·u2002Islet transplantation is a promising technique for treating patients with diabetes. The liver is the current site of choice for clinical islet transplantation, but the major disadvantage of islet transplantation in this site is that many intraportal grafts are lost or damaged in the early stage of transplantation [1, 2].Additional disadvantages include the difficulty of monitoring the islets ...
Get Price2021-4-6u2002·u2002The cells were grown in RPMI 1640 medium (HyClone, Logan, UT) supplemented with 10% (v/v) fetal bovine serum, 100 U/ml of penicillin, and 100 mg/ml of streptomycin at 37 °C in 5% (v/v) CO 2 atmosphere. The uptake of ATV and GCDCA-S were measured using ATV and GCDCA-S (10 µM) in the presence or absence of RIF (0.1, 0.5, 1, 5, 10 and 50 µM).
Get Price2019-5-13u2002·u2002human fetal liver (FL), cord blood (CB), and adult bone marrow (BM) enhance CD34+ hematopoietic progenitor cell proliferation and erythropoiesis [19]. We have exam-ined the growth and erythroid differentiation capacity of CD34+ cells only, already committed toward hematopoiesis in fetal and adult hematopoietic cells.
Get Price2019-9-10u2002·u2002Cholestatic liver diseases are characterized by impaired bile formation, resulting in hepatic accumulation of BA, cholesterol, and bilirubin 24; once BAs accumulate in high concentrations, they can leak from canaliculi and bile ducts, thus causing hepatic cell death, inflammation, fibrosis, and cancer. 25 Cholestatic liver diseases are also ...
Get Price2016-3-18u2002·u2002MCF-7 cells were cultured in Dulbecco's modified eagle medium (DMEM) supplemented with 10% fetal bovine serum (FBS) and 1% of penicillin and streptomycin. For in vitro detection of ROS generation, MCF-7 cells were incubated with DCFH-DA (10 μM) along with INPs or I-ARCs for 30 min, according to the manufacturer's suggested protocol.
Get Price2021-9-19u2002·u2002Hepatocyte seeding and culture medium. William's medium E containing 10 % fetal bovine serum (FBS), suitable antibiotics and 2 ng/mL insulin. Media should be warmed to 37 °C before use (see Note 1). Phosphate-buffered saline (PBS). 137 mM NaCl, 2.7 mM KCl, 10 mM Na 2 HPO 4 ·2H 2 O, 1.8 mM KH 2 PO 4 in deionized water. Adjust to pH 7.4 and ...
Get Price2016-3-18u2002·u2002In conclusion, we demonstrated our primary goal: the necessity of sufficient oxygen supply by Hb for efficient PDT against hypoxic tumors, and the self-monitoring PDT through FL/PA dual-modal imaging.
Get Price2011-1-6u2002·u2002Fibrinogen is a hexamer composed of 2 copies of 3 polypeptide chains Aα, Bβ and γ. These are encoded by 3 genes, FGB, FGA, and FGG (ordered from centromere to telomere) clustered in a 50-kb region on the long arm of human chromosome 4 (4q32). Fibrinogen biosynthesis occurs in the liver where the 3 genes undergo coordinate transcription.
Get Price2008-10-1u2002·u2002The cells were plated at a concentration of 10 5 cells/mL in Dulbecco's modified Eagle's medium with 10% fetal bovine serum, 2 mM L-glutamine, 100 units/mL penicillin, 10 µg/mL streptomycin (Invitrogen). Twenty percent fetal bovine serum was used for …
Get Price2021-1-1u2002·u2002The liver is one of the few solid organs with a high regeneration capacity. After partial hepatectomy (PHx) or chemical challenge in the mammalian liver, almost all remnant hepatocytes rapidly initiate mitosis to generate new cells, and the liver can be reconstituted within 1 week. 1, 2 Many genes and signaling pathways are involved in this process. . For example, immediately …
Get Price2020-2-15u2002·u2002Islet transplantation is a promising technique for treating patients with diabetes. The liver is the current site of choice for clinical islet transplantation, but the major disadvantage of islet transplantation in this site is that many intraportal grafts are lost or damaged in the early stage of transplantation [1, 2].Additional disadvantages include the difficulty of monitoring the islets ...
Get Price2021-4-6u2002·u2002The cells were grown in RPMI 1640 medium (HyClone, Logan, UT) supplemented with 10% (v/v) fetal bovine serum, 100 U/ml of penicillin, and 100 mg/ml of streptomycin at 37 °C in 5% (v/v) CO 2 atmosphere. The uptake of ATV and GCDCA-S were measured using ATV and GCDCA-S (10 µM) in the presence or absence of RIF (0.1, 0.5, 1, 5, 10 and 50 µM).
Get Price2019-5-13u2002·u2002human fetal liver (FL), cord blood (CB), and adult bone marrow (BM) enhance CD34+ hematopoietic progenitor cell proliferation and erythropoiesis [19]. We have exam-ined the growth and erythroid differentiation capacity of CD34+ cells only, already committed toward hematopoiesis in fetal and adult hematopoietic cells.
Get Price2019-9-10u2002·u2002Cholestatic liver diseases are characterized by impaired bile formation, resulting in hepatic accumulation of BA, cholesterol, and bilirubin 24; once BAs accumulate in high concentrations, they can leak from canaliculi and bile ducts, thus causing hepatic cell death, inflammation, fibrosis, and cancer. 25 Cholestatic liver diseases are also ...
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