argentina good-smelling fetal bovine serum replacement direct manufacturers

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  • united states affordable newborn calf serum replacement ...

    Fetal Bovine Serum, qualified, heat inactivated, United States Gibco fetal bovine sera offer excellent value for basic cell culture, specialty research, and specific assays, earning the trust of researchers with consistent quality and award-winning support that helps meet your research needs and budget requirementsSera Category: Value Plus ...

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  • Human Serum is as Efficient as Fetal Bovine Serum in ...

    2011-5-21u2002·u2002a Effect of serum type on proliferation of hMSC-TERT cells. hMSC-TERT cells were cultured in the presence of SFM (serum free), 2.5%, 5% and 10% HuS (human serum) and 10% FBS (fetal bovine serum). Results are presented as mean ± SEM of 3 independent experiments, b Long-term growth curves of hMSC-TERT cells cultured in FBS (10%) and HuS (5%) showing cumulative population doublings …

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  • Human serum and platelet lysate are appropriate xeno

    2018-5-2u2002·u2002Because the current use of fetal bovine serum (FBS) to isolate and expand hMuStem cells raises several ethical, safety, and supply concerns, we assessed the use of two alternative xeno-free blood derivatives: human serum (HS) and a human platelet lysate (hPL). hMuStem cells were isolated and expanded in vitro in either HS-supplemented or hPL ...

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  • (PDF) A Severe Case of Fraudulent Blending of Fetal Bovine ...

    Fetal bovine serum (FBS) is a widely used growth supplement in the in vitro culturing of animal and human cells, tissues and organs, notably due to the occurrence of abundant micro- and ...

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  • Serum and supplement optimization for EU

    2014-1-20u2002·u2002Culture standardization according to Good Manufacturing Practices (GMPs) is a mandatory step for clinical translation. One of the main issues raised is the use of xenogenic additives (e.g. FBS, foetal bovine serum) in cell culture media, which carries the risk of contamination with infectious viral/prion agents, and the possible induction of ...

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  • Oxidative degradation of polyamines by serum

    2018-7-10u2002·u2002For samples, firstly, different concentrations of spermine or spermidine (0, 10, 20, 40, 60 and 80 μM) were incubated with different serums (calf serum, bovine serum, fetal bovine serum, goat ...

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  • Anti-SOX4 antibody [154C4a] (ab70598)

    2021-7-29u2002·u2002ab70598 was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum and filtered through a 0.22µm membrane.

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  • Friction and wear performance evaluation of UHMWPE

    2020-1-1u2002·u2002A continuous linear reciprocating motion pin on disc (POD) tribo-meter (shown in Fig. 2) was used to perform the friction and wear tests in biological lubricants at body temperature (37±1 °C).The normal load was taken as 39 N, 46 N and 52 N. The duration of the test was 60 min and the frequency was kept constant at 5 Hz, respectively.

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  • The Replacement of Fetal Bovine Serum with Bovine Serum ...

    2020-6-12u2002·u2002Bovine serum albumin (BSA) is a source of protein that can be used as an alternative to embryo culture, considering that free fetal bovine serum (FBS) systems increase embryo cryotolerance. 18 Although there have been several studies using BSA supplementation in embryo culture media, 20,21 studies carried out with both steps, in vitro ...

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  • Human Serum is as Efficient as Fetal Bovine Serum in ...

    2011-5-21u2002·u2002a Effect of serum type on proliferation of hMSC-TERT cells. hMSC-TERT cells were cultured in the presence of SFM (serum free), 2.5%, 5% and 10% HuS (human serum) and 10% FBS (fetal bovine serum). Results are presented as mean ± SEM of 3 independent experiments, b Long-term growth curves of hMSC-TERT cells cultured in FBS (10%) and HuS (5%) showing cumulative …

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  • Mesenchymal stem cells cultured in serum-free medium ...

    2021-3-23u2002·u2002Mesenchymal stem cells (MSCs) provide potential treatments for peritoneal fibrosis. However, MSCs cultured in media containing serum bring risks of infection and other problems. In this study, we compared the effect of human MSCs in serum-free medium (SF-MSCs) on peritoneal fibrosis with that of MSCs cultured in medium containing 10% fetal bovine serum (10%MSCs).

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  • Frontiers

    The cells will start to divide after they are cultured in an appropriate culture medium, which will provide nutrients, hormones and growth factors. The best medium is known to contain fetal bovine serum (FBS), a serum made from the blood of a dead calf, which is going to be rate-limiting, and not acceptable for vegetarians nor vegans.

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  • Anti-SOX4 antibody [154C4a] (ab70598)

    2021-7-29u2002·u2002ab70598 was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum and filtered through a 0.22µm membrane.

    Get Price
  • Resveratrol enhances pluripotency of mouse embryonic stem ...

    2019-2-4u2002·u2002HEK293T cells were maintained in DMEM (Biolot) supplemented with 10% fetal bovine serum (HyClone) at 37 °C in an atmosphere of 5% CO 2. Resveratrol purchased from Calbiochem was added to the ...

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  • Structural analysis reveals TLR7 dynamics underlying ...

    2020-10-15u2002·u2002The cells re-seeded into 96-well plate (AGC techno glass) at 2 × 10 4 cells/well in DMEM (GIBCO) with 10% fetal bovine serum (GIBCO), …

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  • Scaled preparation of extracellular vesicles from ...

    A critical aspect in the EV field is the evaluation procedure for EV preparation methods. Currently, the minimal information for studies of extracellular vesicles 2018 (MISEV2018) guidelines of the International Society of Extracellular vesicle (ISEV) recommend to characterize obtained EV preparations by particle quantification technologies, e.g., by nanoparticle tracking analysis …

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  • Simplification of culture conditions and feeder-free ...

    2021-5-26u2002·u2002Bovine embryonic stem cells (bESCs) extend the lifespan of the transient pluripotent bovine inner cell mass in vitro. After years of research, …

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  • Anti-LYVE1 antibody (ab36993)

    Asked on Mar 25 2013. Answer. ab36993's immunogen is a recombinant full length protein produced in insect cells, corresponding to amino acids 24-232 of Human LYVE1 and is fused to a C-terminal His-tag (6xHis). This antibody is therefore not …

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  • Human Serum is as Efficient as Fetal Bovine Serum in ...

    2011-5-21u2002·u2002a Effect of serum type on proliferation of hMSC-TERT cells. hMSC-TERT cells were cultured in the presence of SFM (serum free), 2.5%, 5% and 10% HuS (human serum) and 10% FBS (fetal bovine serum). Results are presented as mean ± SEM of 3 independent experiments, b Long-term growth curves of hMSC-TERT cells cultured in FBS (10%) and HuS (5%) showing cumulative population doublings …

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  • Hsa-miR-100-3p Controls the Proliferation, DNA Synthesis ...

    2021-5-11u2002·u2002We revealed that miR-100-3p was expressed at a higher level in human Sertoli cells by 10% fetal bovine serum (FBS) than 0.5% FBS. MiR-100-3p mimics enhanced the DNA synthesis and the proliferation of human Sertoli cells, as indicated by 5-ethynyl-2′-deoxyuridine (EdU) and Cell Counting Kit-8 (CCK-8) assays.

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  • Biochemical markers for pregnancy in the spent culture ...

    2021-5-12u2002·u2002They were placed in polystyrene tubes containing 400 μL of maturation medium [TCM-199 with Earl's salts (Gibco®, Burlington, Canada) supplemented with 0.075 mg/mL amikacin, 10% fetal bovine serum (FBS) (Gibco®), 0.1 mg/mL of L-glutamine, 1 μM of pyruvate, 0.1 mM of cysteamine, and 0.01 IU/mL of FSH], and covered with 250 μL of mineral oil.

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  • Platelet lysate as a substitute for animal serum for the ...

    2016-7-13u2002·u2002The use of fetal bovine serum (FBS) as a cell culture supplement is discouraged by regulatory authorities to limit the risk of zoonoses and xenogeneic immune reactions in the transplanted host. Additionally, FBS production came under scrutiny due to animal welfare concerns. Platelet derivatives have been proposed as FBS substitutes for the ex-vivo expansion of mesenchymal …

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  • Comparison of biological and transcriptomic effects of ...

    Human lung epithelial cells (BEAS-2B) were purchased from Beyotime (Shanghai, China), and cultured in RPMI 1640 containing 10% (v: v) fetal bovine serum and 10 U/ml of penicillin-streptomycin in a humidified incubator at 37 °C with 5% CO 2 atmosphere. Cells were split every 2–3 days. 2.3.

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  • Comparative immunogenicity of decellularized wild type

    2021-9-1u2002·u2002Primary monocytes were routinely cultured in RPMI (GIBCO) with 2 mM l-glutamine and 10% fetal bovine serum (FBS) and split into two groups, one left undifferentiated and the other differentiated into macrophages by culturing in media supplemented with 100 nM phorbol myristate acetate (PMA) for 48 h. To obtain cells from leukapheresis filters ...

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  • Control of mesenchymal cell fate via application of FGF-8b ...

    2021-3-1u2002·u2002Fetal bovine serum (FBS, 16000044), horse serum (16050122), penicillin-streptomycin (pen/strep, 15140122), and sodium pyruvate (11360070) were obtained from Gibco, Thermo Fisher Scientific (Waltham, Massachusetts). 2.2. Adipose derived stem cells (ADSCs) and muscle progenitor cells (MPCs) isolation and culture

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  • The biotechnological potential of whey

    2016-8-19u2002·u2002Fetal bovine serum is used in mammalian cell culture, used in the manufacture of vaccines and biopharmaceuticals. The use of a whey derived growth factor media would provide a cheaper and safer alternative to fetal bovine serum that would not be at risk of contamination from mycoplasma, viruses and Bovine Spongiform Encephalopathy (BSE) (Keenan ...

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  • Large-scale production of functional human serum

    2011-11-22u2002·u2002Human serum albumin (HSA) is widely used in clinical and cell culture applications. Conventional production of HSA from human blood is limited by the availability of blood donation and the high risk of viral transmission from donors. Here, we report the production of Oryza sativa recombinant HSA (OsrHSA) from transgenic rice seeds. The level of OsrHSA reached 10.58% of the total soluble ...

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  • Anti-LYVE1 antibody (ab36993)

    Asked on Mar 25 2013. Answer. ab36993's immunogen is a recombinant full length protein produced in insect cells, corresponding to amino acids 24-232 of Human LYVE1 and is fused to a C-terminal His-tag (6xHis). This antibody is therefore not specifically directed towards the C-terminal of LYVE-1.

    Get Price
  • Lonza, Enabling a Healthier World

    About Lonza. At Lonza, we enable a healthier world by supporting our healthcare customers on the path to commercialization. Our community of 15,000 skilled employees work across a global network of more than 30 sites to deliver for our customers across the pharma, biotech and nutrition markets. By combining technological insight with world ...

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  • Serum and supplement optimization for EU GMP‐compliance in ...

    2014-1-20u2002·u2002Culture standardization according to Good Manufacturing Practices (GMPs) is a mandatory step for clinical translation. One of the main issues raised is the use of xenogenic additives (e.g. FBS, foetal bovine serum) in cell culture media, which carries the risk of contamination with infectious viral/prion agents, and the possible induction of ...

    Get Price
  • Comparison of biological and transcriptomic effects of ...

    Human lung epithelial cells (BEAS-2B) were purchased from Beyotime (Shanghai, China), and cultured in RPMI 1640 containing 10% (v: v) fetal bovine serum and 10 U/ml of penicillin-streptomycin in a humidified incubator at 37 °C with 5% CO 2 …

    Get Price
  • Simplification of culture conditions and feeder-free ...

    2021-5-26u2002·u2002Bovine embryonic stem cells (bESCs) extend the lifespan of the transient pluripotent bovine inner cell mass in vitro. After years of research, …

    Get Price
  • Corneal Repair with Adhesive Cell Sheets of Fetal ...

    2021-1-7u2002·u2002Background: Corneal scarring or disease may lead to severe corneal opacification and consequently, severe loss of vision due to the complete loss of corneal epithelial cells. We studied the use of epithelial cell sheets differentiated from fetal cartilage-derived stem cells (FCSC) to resurface damaged cornea. Methods: The FCSC were isolated from the femoral head of …

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  • Addressing the Manufacturing Challenges of Cell-Based ...

    2019-12-17u2002·u2002Abstract. Exciting developments in the cell therapy field over the last decades have led to an increasing number of clinical trials and the first cell products receiving marketing authorization. In spite of substantial progress in the field, manufacturing of cell-based therapies presents multiple challenges that need to be addressed in order to ...

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  • Research & Biopharmaceutical Manufacturing

    We offer solutions to support your work from biological research to clinical therapy, including tools for research, drug discovery, diagnostics and bioprocessing.

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  • Structural analysis reveals TLR7 dynamics underlying ...

    2020-10-15u2002·u2002The cells re-seeded into 96-well plate (AGC techno glass) at 2 × 10 4 cells/well in DMEM (GIBCO) with 10% fetal bovine serum (GIBCO), …

    Get Price
  • VWR, Part of Avantor - Chemicals and Laboratory Scientific ...

    VWR, Part of Avantor - Chemicals and Laboratory Scientific Supplies. VWR, Part of Avantor. Carousel. Moving Forward. Together. So much has changed during this unprecedented time, except your ability to count on Avantor. We continue to set science in motion, and know safety is paramount for you and your colleagues.

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  • The biotechnological potential of whey

    2016-8-19u2002·u2002The main problem associated with whey comes from its potential to damage the environment. It has a very high Biochemical Oxygen Demand (BOD) that can vary from 40,000 to 60,000 mg/L and a very high Chemical Oxygen Demand (COD) of between 50,000 and 80,000 mg/L (Chatzipaschali and Stamatis 2012).The waste load of whey is equivalent to that of 100–175 …

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  • Research & Biopharmaceutical Manufacturing

    We offer solutions to support your work from biological research to clinical therapy, including tools for research, drug discovery, diagnostics and bioprocessing.

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  • Comparison of biological and transcriptomic effects of ...

    Human lung epithelial cells (BEAS-2B) were purchased from Beyotime (Shanghai, China), and cultured in RPMI 1640 containing 10% (v: v) fetal bovine serum and 10 U/ml of penicillin-streptomycin in a humidified incubator at 37 °C with 5% CO 2 atmosphere. Cells were split every 2–3 days. 2.3.

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  • Mechanistic Study on the Reduction of SWCNT-induced ...

    SWCNTs coated by bovine serum albumin (BSA) imposed no obvious toxicity to cells. Albumin is chosen for protection of SWCNTs because it is the most abundant protein in the circulation system for the sake of in vivo applications of SWCNTs, as well as in the cell culture media used during in vitro study. [ 19 ]

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  • Structural analysis reveals TLR7 dynamics underlying ...

    2020-10-15u2002·u2002The cells re-seeded into 96-well plate (AGC techno glass) at 2 × 10 4 cells/well in DMEM (GIBCO) with 10% fetal bovine serum (GIBCO), and 10 μg ml −1 Blastcidin S HCl (GIBCO) and then ...

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  • ARID1A regulates R-loop associated DNA replication stress

    2021-4-7u2002·u2002RMG1 cells were cultivated in RPMI-1640 medium (Stemcell technologies) supplemented with 10% fetal bovine serum (Life Technologies) in 5% CO 2 at 37°C. ARID1A was knocked out in RMG1 cells using CRISPR/cas9 technology with gRNA targeting exon 2 of ARID1A gene (5'-CTTGCTGCGGTCCTGACGGAGG-3').

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  • Frontiers

    2021-5-26u2002·u2002Humanized bone marrow-liver-thymus (HuBLT) mice are a revolutionary small-animal model that has facilitated the study of human immune function and human-restricted pathogens, including human immunodeficiency virus type 1 (HIV-1). These mice recapitulate many aspects of acute and chronic HIV-1 infection, but exhibit weak and variable T-cell responses when challenged with HIV-1, …

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  • Addressing the Manufacturing Challenges of Cell-Based ...

    2019-12-17u2002·u2002Abstract. Exciting developments in the cell therapy field over the last decades have led to an increasing number of clinical trials and the first cell products receiving marketing authorization. In spite of substantial progress in the field, manufacturing of cell-based therapies presents multiple challenges that need to be addressed in order to ...

    Get Price
  • VWR, Part of Avantor - Chemicals and Laboratory Scientific ...

    VWR, Part of Avantor - Chemicals and Laboratory Scientific Supplies. VWR, Part of Avantor. Carousel. Moving Forward. Together. So much has changed during this unprecedented time, except your ability to count on Avantor. We continue to set science in motion, and know safety is …

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  • How algae's potential could make other ingredients green ...

    2021-1-21u2002·u2002Major manufacturers, ... has long been expensive because of the need for fetal bovine serum to create a cell growth medium. ... Spicer said Algenuity hopes to offer some direct-to-consumer ...

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  • Fume Hoods and Enclosures

    Avantor ®, a Fortune 500 company, is a leading global provider of mission-critical products and services to customers in the biopharma, healthcare, education & government, and advanced technologies & applied materials industries.Our portfolio is used in virtually every stage of the most important research, development and production activities in the industries we serve.

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  • argentina cedar essential oil purifies skin direct ...

    The primary component in coriander seed essential oil is linalool. This constituent makes up about 40% to 85% of the essential oil. It just depends on the country and manufacturers. While the oil from Argentina contains about 69% to 87% linalool, the oil from Iran usually contains from …

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  • Addressing the Manufacturing Challenges of Cell-Based ...

    2019-12-17u2002·u2002Abstract. Exciting developments in the cell therapy field over the last decades have led to an increasing number of clinical trials and the first cell products receiving marketing authorization. In spite of substantial progress in the field, manufacturing of cell-based therapies presents multiple challenges that need to be addressed in order to ...

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  • Clinique

    2021-10-2u2002·u2002Shop the official Clinique website for skincare, makeup, fragrances and gifts. Read reviews and get free shipping today. Allergy tested. Dermatologist tested. 100% fragrance free. Get tips, advice and inspiration anytime you need it with our team of Skin Services experts.

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  • Research & Biopharmaceutical Manufacturing

    We offer solutions to support your work from biological research to clinical therapy, including tools for research, drug discovery, diagnostics and bioprocessing.

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  • Pernod Ricard, créateurs de convivialité

    2021-10-2u2002·u2002We are 'créateurs de convivialité' Our ambition is to turn every social interaction into a genuine and friendly experience of sharing. As we create and share our Pernod Ricard products we become immersed in this extraordinary human experience of convivialité. We are respectful and responsible hosts, committed to nurturing every terroir and its biodiversity.

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  • Powertec Outboards

    The Powertec generators are the best option in the power system solutions for boats. Fuel Water Filter Protect your boat's fuel system with our wide selection of direct replacement OEM fuel system.

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  • Uflow Solenoid Valve

    solenoid valve manufacturers India, angle seat valve manufacturers India,uflow solenoid valve, Unifine Engineering, control valve, pneumatic control valve, actuated Butterfly Valve suppliers, actuated ball valve suppliers, Single double Pneumatic Actuator Pneumatic products solenoid valve suppliers worldwide global

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  • Pilkington Automotive

    Operating as a single global organization serving the Original Equipment (OE) and Local Automotive Glass Replacement (AGR) Aftermarket sectors Featured Products and Services Automotive Glass Replacement

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  • Jinming Toys: Shantou Toys Suppliers toys Manufacturers ...

    2021-9-25u2002·u2002Jinming Toys & Crafts I/E Co., Ltd. is one of the largest toy suppliers in China. We specialized in exporting plastic toys, crafts, gifts, and have more than Millions kinds of …

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    Be where your customers are! Promote and market your services. Receive direct quote requests. Back office support, set up regional offices and much more... JOIN NOW - IT´S FREE! If your business is Trade Shows, Customs, Trade Finance, Inspection, Insurance, Trade Laws, Trade Solutions, Translation or involves in foreign trade supply chain, you ...

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